In order to develop antitumor agent which indicates weak side effects and strong antitumor activity, i cytotoxicity of Methyl gallate (MG) was evaluated by MTT assay and SRB assay of colorimetric assay
methods on the cultured NIH 3T3 fibroblasts, human oral epithelioid carcinoma cells (KB cells) and human skin melanoma cells (SK-MEL-3 Cells). NIH 3T3 fibroblast, KB cells and SK-MEL-3 cells were cultured in EMEM and RPMI 1640 media containing 10 % fetal bovine serum, antibiotics and fungizone. After incubation for 24 hrs, the cells were treated with MG by dose dependent manner for 48 hrs under the same condition.
The MTT and SRB quantity were measured by ELISA reader (Spectra Max 250, USA). The microscopic study was carried out to observe morphological changes of cultured NIH 3T3 cells, KB cells and SK-MEL-3 cells.
The results were as follows;
1. The SRBso of human lung tumor cells, human ovarian tumor cells, human melanoma tumor cells, human colon tumor cells and human CNS tumor cells treated with MG was 69.80 u M, 44.90 u M, 22.12 u M, 29.24 u M and 59.29 u M, respectively.
2. The MTTso and SRBso in NIH 3T3 cells treated with MG were 16.67mM and 8.7 mM, respectively.
3. The MTTm and SRB5o in KB cells treated with MG were 65.55 u M and 103.90 j UM, respectively.
4. The MTTm and SRBso in SK-MEL-3 cells treated with MG were 168.81 u M and 254.35 u M,
respectively.
5. Morphological change of cultured NIH 3T3 cells treated with MG was weak, but KB cell treated with MG was severe.
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